当期Cell Stem Cell封面：一个橙子慢慢变成苹果，象征着细胞重编程过程
诱导多能性干细胞（induced pluripotent stem cells，iPS cells）是具有胚胎干细胞特性的一种细胞，由体细胞诱导而来，可作为胚胎干细胞最好的替代品用于科学研究和临床治疗。但是iPS细胞的成功诱导比较复杂，诱导效率一直是科学界的难题，通常每万个细胞才能诱导成功一例iPS细胞。
最近，中科院广州生物医药与健康研究院裴端卿带领的研究小组发现，通过在培养过程中添加维生素C可使iPS诱导效率提高10倍。通过老鼠和人细胞实验发现，培养时添加维生素C可促进相关基因表达，推动体细胞进入重编程状态。
该项成果将于美国时间今年12月24日在世界干细胞权威杂志《细胞—干细胞》（Cell Stem Cell）在线发布，并且被选为封面文章。（生物谷Bioon.com）
提高iPS诱导效率方式：
Nature：iPS细胞转化率提高约百倍
Nature Methods：新方法可提高成体组织转化iPS细胞效率
Nature：高效诱导iPS的方法模型
Cell Stem Cell：两种因子可提高iPS转化率
Nature Biotechnology：新方法大幅提高IPS生产率
Cell Stem Cell：低氧环境可提高培养iPS细胞效率
各种诱导iPS方法：
Cell Stem Cell：利用重组蛋白诱导iPS细胞成功
Cloning and Stem Cells：通过控制培养条件可激发体细胞的干细胞特性
Science：不含外来DNA的人类iPS细胞
Nature：不使用病毒载体生成人iPS细胞系的方法
Cell Stem Cell：化学小分子可替代基因诱导iPS细胞
Cell：利用单基因调控诱导iPS
生物谷推荐原始出处：
Cell Stem Cell, 24 December 2009 doi:10.1016/j.stem.2009.12.001
Vitamin C Enhances the Generation of Mouse and Human Induced Pluripotent Stem Cells
Miguel Angel Esteban1, 6, Tao Wang1, 6, Baoming Qin1, 6, Jiayin Yang1, Dajiang Qin1, Jinglei Cai1, Wen Li1, Zhihui Weng1, Jiekai Chen1, Su Ni1, Keshi Chen1, Yuan Li1, Xiaopeng Liu1, Jianyong Xu1, Shiqiang Zhang1, Feng Li1, Wenzhi He1, Krystyna Labuda2, Yancheng Song3, Anja Peterbauer4, Susanne Wolbank2, Heinz Redl2, Mei Zhong5, Daozhang Cai3, Lingwen Zeng1 and Duanqing Pei1, ,
1 Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China
2 Ludwig Boltzmann Institute for Clinical and Experimental Traumatology, Austrian Cluster for Tissue Regeneration, Vienna 1200, Austria
3 The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, China
4 Red Cross Blood Transfusion Service of Upper Austria, Austrian Cluster for Tissue Regeneration, Linz 4020, Austria
5 Center for Prenatal and Hereditary Disease Diagnosis, Nanfang Hospital, Guangzhou 510515, China
Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. However, the low efficiency and slow kinetics of the reprogramming process have hampered progress with this technology. Here we report that a natural compound, vitamin C (Vc), enhances iPSC generation from both mouse and human somatic cells. Vc acts at least in part by alleviating cell senescence, a recently identified roadblock for reprogramming. In addition, Vc accelerates gene expression changes and promotes the transition of pre-iPSC colonies to a fully reprogrammed state. Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process.? Vitamin C improves the speed and efficiency of mouse iPSC generation . Adding vitamin C converts pre-iPSCs to iPSCs . Vitamin C alleviates the senescence roadblock to reprogramming . Human iPSC generation is also improved by vitamin C
