使用全基因组寡核苷酸微阵列调查LYP9 水稻转录组谱及其亲本，中科院遗传所朱立煌、农科院以及国家杂交水稻工程技术研究中心的科学家研究了一种超级杂交水稻品种的遗传背景和分子机制。这种水稻由中国杂交水稻之父袁隆平在上世纪70年代开发，名为LYP9，是解决中国粮食问题的水稻品种之一。
聚类分析表明F1代杂交种表达谱汇集并超越了亲本的优势，从22266个表达基因中发现了7078个基因均被7个样本表达。经过两亲本（DGPP）之间比较和杂交种与亲本之间比较（DGHP）表明，DGHP的能量代谢与转运基因表达要多于DGPP。这些差异表达基因，尤其是参与碳水化合物代谢的基因，可能是控制杂种优势的候选者。（生物谷Bioon.com）
生物谷推荐原始出处：
PNAS April 16, 2009, doi: 10.1073/pnas.0902340106
A transcriptomic analysis of superhybrid rice LYP9 and its parents
Gang Weia,b,1, Yong Taoa,b,1, Guozhen Liuc,d,1, Chen Chenc, Renyuan Luob, Hongai Xiaa, Qiang Gana,b, Haipan Zengc, Zhike Luc, Yuning Hanc, Xiaobing Lia, Guisheng Songa, Hongli Zhaia, Yonggang Penga, Dayong Lia, Honglin Xua, Xiaoli Weia, Mengliang Caoe, Huafeng Denge, Yeyun Xine, Xiqin Fue, Longping Yuane,2, Jun Yuc,2, Zhen Zhua,2 and Lihuang Zhua,2
aState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
bGraduate School of the Chinese Academy of Sciences, Beijing 100039, China;
cKey Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China;
dCollege of Life Sciences, Hebei Agricultural University, Baoding 071001, China; and
eNational Hybrid Rice Research and Development Center, Changsha 410125, China
By using a whole-genome oligonucleotide microarray, designed based on known and predicted indica rice genes, we investigated transcriptome profiles in developing leaves and panicles of superhybrid rice LYP9 and its parental cultivars 93-11 and PA64s. We detected 22,266 expressed genes out of 36,926 total genes set collectively from 7 tissues, including leaves at seedling and tillering stages, flag leaves at booting, heading, flowering, and filling stages, and panicles at filling stage. Clustering results showed that the F1 hybrid's expression profiles resembled those of its parental lines more than that which lies between the 2 parental lines. Out of the total gene set, 7,078 genes are shared by all sampled tissues and 3,926 genes (10.6% of the total gene set) are differentially expressed genes (DG). As we divided DG into those between the parents (DGPP) and between the hybrid and its parents (DGHP), the comparative results showed that genes in the categories of energy metabolism and transport are enriched in DGHP rather than in DGPP. In addition, we correlated the concurrence of DG and yield-related quantitative trait loci, providing a potential group of heterosis-related genes.
