栽培种落花生(Arachis hypogaea L.)是世界上第4大油料作物,它主要生长于热带、亚热带和暖温带地区。非洲和亚洲贫瘠地区的落花生生产面临多种生物及非生物胁迫的挑战。而分子标记及遗传图谱是利用分子育种方法来抵制这些生物及非生物胁迫的必要前提。对于落花生而言,尽管目前已经开发出了数百种分子标记(例如微卫星标记或简单重复序列/SSR标记),并且在二倍体野生种或人工四倍体种作图群体的基础上得到了遗传图谱,但是目前还没有有关栽培落花生的遗传图谱。
国际半干旱热带作物研究所(ICRISAT)的一组科学家与巴西EMBRAPA/天主教大学、美国乔治亚大学和塔斯基吉大学的同事共同绘制出首例栽培种落花生SSR遗传连锁图谱。这一图谱包含135个SSR标记,22个连锁群。该研究小组已经证明了这一遗传图谱在栽培种落花生性状定位及豆类基因比较作图中的应用。(生物谷Bioon.com)
生物谷推荐原始出处:
Theor Appl Genet (2009) 118:729–739 DOI 10.1007/s00122-008-0933-x
The first SSR-based genetic linkage map for cultivated groundnut(Arachis hypogaea L.)
R. K. Varshney ?D. J. Bertioli ?M. C. Moretzsohn ?V. Vadez L. Krishnamurthy ?R. Aruna ?S. N. Nigam ?B. J. Moss ?K. Seetha K. Ravi ?G. He ?S. J. Knapp ?D. A. Hoisington
Abstract Molecular markers and genetic linkage maps are pre-requisites for molecular breeding in any crop species.In case of peanut or groundnut (Arachis hypogaea L.),an amphidiploid (4X) species, not a single genetic map is,however, available based on a mapping population derived from cultivated genotypes. In order to develop a genetic linkage map for tetraploid cultivated groundnut, a total of 1,145 microsatellite or simple sequence repeat (SSR) markers available in public domain as well as unpublished markers from several sources were screened on two genotypes, TAG 24 and ICGV 86031 that are parents of a recombinant inbred line mapping population. As a result,