重点实验室最近在专业期刊《Food and Chemical Toxicology》杂志上发表一篇题为“A BALB/c mouse model for assessing the potential allergenicity of proteins： Comparison of allergen dose，sensitization frequency，timepoint and sex”的学术论文。
随着转基因作物的快速发展，转基因食品的安全性成为广大民众关心的问题，转基因食品引起食物过敏的可能性是人们关注的焦点之一。BALB/c小鼠是近交高IgE应答品系，优先产生Th2 型免疫应答和IgE抗体，常被用来判定蛋白质的潜在致敏性。
本研究选用造模致敏原卵清蛋白（OVA），在本实验室前期初步建立的BALB/c小鼠致敏性评价动物模型基础上，对BALB/c小鼠腹腔注射致敏动物模型进行了完善。从致敏原剂量、致敏频次、致敏周期以及动物性别四个方面进行研究。选用OVA特异性IgE和IgG1（OVA sIgE， sIgG1）作为判定终点。
综合考虑OVA特异性IgE和IgG1的结果表明，在试验第0、3、6、9、12天进行5次腹腔注射比第0、7天进行两次2次腹腔注射能激发产生更高的OVA特异性IgE和IgG1，致敏周期宜选用28天，而性别则对致敏性无影响。同时，我们进一步对腹腔注射5次，每次给予0.05mg OVA的BALB/c小鼠在第28天取脾，进行脾细胞培养。用0.5 mg/ml OVA激发脾细胞两天后，检测细胞培养上清液中的细胞因子（IL-4和IFN-γ）。结果表明，试验组IL-4含量显著高于对照组，试验组IFN-γ含量与对照组相比无显著变化。依据本研究结果，在试验第0、3、6、9、12天腹腔注射5次给予BALB/c小鼠0.05mg OVA，在试验第28天取血检测OVA特异性 IgE和IgG1，可建立较理想的BALB/c小鼠蛋白潜在致敏性评价动物模型。
目前，使用动物模型评价转基因作物的致敏性，是国际上研究和开发的热点领域。本研究为转基因食品潜在致敏性蛋白的评价提供了一个更完善的BALB/c小鼠动物模型。为其能够更好地应用于转基因食品的致敏性评价体系中奠定了更坚实的基础。（生物谷Bioon.com）
生物谷推荐的英文摘要
Food and Chemical Toxicology doi.org/10.1016/j.fct.2013.08.004
A BALB/c mouse model for assessing the potential allergenicity of proteins： Comparison of allergen dose， sensitization frequency， timepoint and sex
Chen Chena， 1， Nana Sunb， 1， Yongning Lib， Xudong Jiab
The present study was to investigate the possibility of using the BALB/c mouse as an animal model for assessing the potential allergenicity of proteins.
Specific IgE and IgG1 against ovalbumin were induced by dosing BALB/c mice via intraperitoneal injection (absence of adjuvant). The effects of various allergen doses (5 mg， 0.5 mg or 0.05 mg OVA)， sensitization times (twice or five times)， timepoints (day 14 or day 28) and sex (male or female) were studied. IL-4， IFN-γ， OVA-specific IgE and IgG1 were measured by enzyme-linked immunosorbent assay (ELISA).
A general finding was that mice treated with 0.05 mg OVA had the highest OVA-specific IgE and IgG1， statistically significant higher specific IgE and IgG1 were observed in groups sensitized five times than twice， OVA-specific IgE and IgG1 on day 28 were statistically higher than day 14， and higher IL-4 was observed in OVA-allergic mice than control mice.
These results demonstrate that the BALB/c mouse model treated with 0.05 mg OVA intraperitoneally on days 0， 3， 6， 9， 12 might be used for further experiments. OVA-specific IgE and IgG1 should be detected on day 28. Further studies including reproducibility and other conditions were required before using the BALB/c mouse model for assessing the potential allergenicity of proteins.
